From: National Research Council Canada

Status: Available

Price (in CAD): $500.00

Description: Reference Material of SARS-CoV-2 spike glycoprotein solution - not for resale, purchasing restrictions in place.

Purchasing restrictions: this material is not to be used for commercial purposes. The purchaser is responsible for obtaining the necessary license(s) for the commercialization of a product which comprises SMT1-1.

Application field(s): Clinical, COVID-19

Analyte group(s): Proteins

Unit: 1.2 mL
(The reference materials are packaged in approximate quantities. Please check quarantine restrictions in your country to ensure smooth transfer of the reference materials from Canada to the destination.)

Ships from: Ottawa

How to order

Ordering and shipping

Prices are quoted in Canadian dollars and subject to change without notice.

Order may be faxed, e-mailed, or submitted via the online order form. An email will be sent to confirm the reception of your order.

Pre-payment may be required for some orders. If pre-payment is required clients will be notified, and confirmation that NRC has received the funds is required before any package will be shipped. NRC accepts payment by cheque, direct deposit (Canada only), wire transfer, and credit card. There is a $20.00 service charge for payment by wire transfer.

Orders which are paid by credit card must be submitted via our Virtual Store. For clients who are required or want to pre-pay for their purchases, the Virtual Store is the fastest way to make an advance payment.

For orders not submitted via the Virtual Store, please note that a $25.00 administration fee will apply. This is in addition to any service charges in the case of a payment by wire transfer.

All orders will be shipped via FedEx, with "Carriage Paid To" (CPT) shipping terms, with the exception of some orders that include products classified as Dangerous Goods in Excepted Quantities when FedEx in unable to deliver and the services of our Freight ForwarderFootnote 1 will be used, or if alternate arrangements have been agreed upon by NRC prior to shipping. For more information on countries FedEx is able to deliver dangerous goods to, please refer to their website.

Ottawa - Shipping fees (when shipping via FedEx)

Value of products in order Canada USA Rest of the world
$0.01 - $500 $15 $20 $40
$500.01 - $1000 $20 $35 $70
$1000.01 - $3000 $25 $55 $100
$3000.01 - $5000 $30 $60 $120
$5000.01 - $8000 $50 $70 $150
$8001+ CustomFootnote 1 CustomFootnote 1 CustomFootnote 1
Value of products in order Canada USA Rest of the world

An email will be sent to the purchaser with the shipment tracking information for their order. Clients are encouraged to monitor the shipping progress for any delays. NRC takes no responsibility for delayed or lost shipments and there is no insurance on packages. Shipments are packaged carefully so that the integrity of the products is maintained. If shipment is not received within a reasonable time, please contact the courier service. Customs, duty charges, and custom broker fees are the responsibility of the customer. Check quarantine restrictions in your country to ensure smooth transfer of CRMs from Canada to the destination.

Product documentation including the Certificate of Analysis, MSDS sheet, and invoice/receipt will be provided to clients inside the shipping box, or emailed in PDF format ahead of shipment if possible.

Store CRMs at recommended temperature as stated in the relevant certificate of analysis.

Terms of Purchase Orders are not accepted. Terms of the NRC Sales Agreement are in effect at all times.

Quality of service

At the National Research Council of Canada Metrology Research Centre, employees of the Certified Reference Materials project are committed to delivering quality services in a cost effective manner. We endeavor to provide the highest service standards and the best possible service to our clients. We aim to ensure your satisfaction, abiding by the following principles:

Accessible, dependable and timely

We will make it easy for you to contact us. Our priority is dependable, prompt and accurate service delivered in a timely, cost-effective manner with your convenience in mind. We will serve you in the official language (English or French) of your choice at our designated locations.

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We want you to understand what services are available and how you can contact us with comments and questions. We will provide clear, comprehensive and accurate billing with a choice of convenient payment options.

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Responsive and committed to improvement

We are responsive to your needs and committed to improvement by seeking your views on how this can be accomplished. We will listen and respond to what you say and you will be treated with respect. We welcome your suggestions and comments to Biotoxins, Inorganic or Organic - they represent our opportunity to improve. Our services will be regularly reviewed and improved upon, incorporating feedback obtained from our clients. And, of course, your cooperation in providing full, accurate and timely information will help us provide you with quality service.

Service Standards

  1. Orders are processed and shipped within 5 business days of receiving all the required information from purchaser – temperature sensitive products may require longer processing time due to carrier shipping schedules

SARS-CoV-2 Spike Glycoprotein Reference Material

SMT1‑1 is a reference material (RM) designed for the development of SARS‑CoV‑2 spike glycoprotein detection methods, as well as an antigen source for use in SARS‑CoV‑2 immunological assays. The majority of available SARS-CoV-2 test kits and vaccines exploit the interaction between the SARS‑CoV‑2 spike glycoprotein and human anti-spike antibodies. Reference values for the molar concentration, mass fraction, and mass concentration of SARS‑CoV‑2 spike protein in SMT1‑1 have been established, as listed in Table 1. Values are provided for the known spike protein sequence region that ignores the glycan, and also the full spike glycoprotein where the mass fraction and mass concentration values include an estimate of the molar mass of the glycan. Information values for the size heterogeneity of SARS‑CoV‑2 spike glycoprotein in SMT1‑1 are provided in Table 2. A unit of SMT1‑1 contains 1.2 mL of SARS‑CoV‑2 spike glycoprotein in Dulbecco's phosphate-buffered saline (DPBS) with 10 mM HEPES sodium salt.

The reference values for SMT1‑1 are based on combined results from two orthogonal methods from data generated at the National Research Council of Canada (NRC). Following acid hydrolysis of the protein, amino acid analysis (AAA) by double isotope dilution liquid chromatography tandem mass spectrometry (LC‑ID‑MS/MS) was employed for value assignment of SARS‑CoV‑2 spike glycoprotein in SMT1‑1. In addition, ultraviolet-visible spectrophotometry (UV‑Vis) based on tryptophan and tyrosine absorbance at 280 nm was also performed. The expanded uncertainty (U) for all values is equal to kuc, where uc is the combined standard uncertainty calculated according to the JCGM Guide [1] and k is the coverage factor of two (k = 2, 95 % confidence interval).

Table 1: Reference values and expanded uncertainties (k = 2, 95 % CI) for SMT1-1
Substance Molar concentration
Mass fraction
Mass concentration
SARS-CoV-2 spike protein (a,b)Table note 1 5.68 ± 0.20 0.807 ± 0.026 0.813 ± 0.028
SARS-CoV-2 spike glycoprotein (a,b)Table note 2 5.68 ± 0.20 1.020 ± 0.076 1.028 ± 0.076

The information values for SMT1‑1 are based on data generated at the NRC. The biologically-active state of SARS-CoV-2 spike glycoprotein is trimeric, consisting of three identical monomeric subunits. Size-exclusion liquid chromatography with UV-Vis detection (LC-SEC-UV) was utilized to determine the size heterogeneity of the SARS-CoV-2 spike glycoprotein in SMT1‑1. The information values in Table 2 can be applied to all of the reference values listed in Table 1.

Table 2: Information values for size heterogeneity of SARS-CoV-2 spike glycoprotein for SMT1-1
Substance Relative amount (%)
trimer (c) 94
high molecular weight species (c) 4
low molecular weight species (c) 2

Intended use

Distributed in 1.2 mL units, this reference material is primarily intended for use in development and validation of SARS‑CoV‑2 immunological tests.


It is recommended that the material be stored in a controlled cold temperature environment such as a freezer at approximately ‒80 °C. Short term storage at ‒20 °C may be acceptable however stability of the material under such conditions has not been rigorously assessed.


Included in the combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-unit variation (uhom), and uncertainties related to stability (ustability). Expressed as standard uncertainties, these components are listed in Table 3.

Table 3: Uncertainty components of reference values for SMT1‑1
Substance Uk=2 uc uchar uhom ustability
SARS-CoV-2 spike glycoprotein molar concentration (µmol/L) 0.20 0.10 0.10 0.00 0.00
SARS-CoV-2 spike protein mass fraction (mg/g) 0.026 0.013 0.013 0.000 0.000
SARS-CoV-2 spike glycoprotein mass fraction (mg/g) 0.076 0.038 0.038 0.000 0.000

Date of issue: January 2021
Date of expiry: January 2022

Product documentation:

CA (PDF, 698 kB)
SDS (PDF, 164 kB)

Certificates of Analysis and Safety Data Sheets are now available online in PDF format. Access is free, but is limited to researchers, students, regulators, clients, and other product stakeholders. To request access, please register.

Contact us

Ottawa CRM Sales Office
Metrology Research Centre
1200 Montreal Road
Building M36, Room 1029
Ottawa, Ontario K1A 0R6
Telephone: 613-993-2359
Fax: 613-993-8915

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